Study on Lavender Essential Oil Chemical Compositions by GC-MS and Improved pGC (2024)

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PMC7397202

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Molecules. 2020 Jul; 25(14): 3166.

Published online 2020 Jul 10. doi:10.3390/molecules25143166

PMCID: PMC7397202

PMID: 32664436

Guangyao Dong,^1,² Xiaohui Bai,^1,² Aoken Aimila,^1,² Haji Akber Aisa,^1,³ and Maitinuer Maiwulanjiang^1,^3,^*

Natalizia Miceli, Academic Editor

Author information Article notes Copyright and License information PMC Disclaimer

Associated Data

Supplementary Materials

Abstract

Lavender essential oil from the aerial parts of Lavandula angustifolia Mill. was analyzed by GC-MS equipped with three capillary columns of different polarities, which were HP-1, HP-5 ms and HP-INNOWax. A total of 40 compounds were identified by GC-MS, accounting for 92.03% of the total essential oil compositions. Nineteen monomers were separated by column chromatography and improved preparative gas chromatography (pGC), six of which could not be retrieved from the NIST 14 (National Institute of Standards and Technology, USA; 14th edition) library database. Fifteen compounds were identified for the first time in lavender essential oil. The improved pGC not only doubled the efficiency but also greatly reduced the cost.

Keywords: lavender, essential oil, improved preparative GC, NMR

1. Introduction

Lavender (Lavandula angustifolia Mill.) is a perennial herb and a member of the Lamiaceae (Labiatae) family that is native to the Mediterranean region and has been grown all over the world because of the huge market for essential oils. Lavender essential oil is widely used in fragrances and commodities including colognes, skin lotions, soaps, food flavorings, perfumes and aromatherapeutic medicines [1]. Lavender is also widely cultivated in China, and its main producing area is in Yili, Xinjiang. The Lavandula genus is divided into 37 varieties according to the shape of the leaves, corolla morphology, calyces and bract in “Lavender: The genus Lavandula” [2]. Only the essential oils of three lavender species (Lavandula angustifolia, Lavandula latifolia and Lavandula hybrid) play an important role in the perfume and cosmetics industry [3]. The essential oils of lavender are mainly produced from glands on the surface of the flowers and the leaves [2].

Although the chemical compositions of lavender essential oil were studied by GC-MS in different literatures [4,5], there is no report on the separation and analysis of lavender essential oil by preparative gas chromatography (pGC), NMR and GC-MS equipped with three capillary columns of different polarities. This study could not only verify experimental results, but also the isolated monomer by pGC is of great help to the study of the activity of the single and composite component of lavender essential oil. The combination of these two methods can effectively solve the problem of identifying the accuracy of essential oil components by GC-MS without reference compounds. Therefore, the study has important analytical significance for identifying essential oil components.

Many reports published have shown lavender essential oil to possess high cholinesterase inhibitory activities [6,7] and other biological activities beneficial to human health, such as being antibacterial, antifungal [8], sedative [9], anti-depressive, effective for burns and insect bites [10], anticancer [11], anti-spasmolytic, anti-inflammatory [12], antioxidant [13], acaricides etc. [14]. The main component of essential oils are terpenoids, which are low-molecular and lipophilic compounds that can easily cross the blood-brain barrier [15]. The compounds separated from lavender essential oil could lay the foundation for subsequent drug research.

2. Results and Discussion

2.1. Chemical Composition of the Essential Oils

The essential oil was extracted by hydrodistillation from the fresh plants of lavender aerial parts, and the mean values of the oil yields were 1.3% (v/w; mL/g) based on fresh weights. The essential oil GC-MS results of three different polarity capillary columns, which were HP-5 ms, HP-1 and HP-INNOWax capillary columns, are presented in Table 1 and Figures S1–S3 (Supplementary Materials). The relative content of the essential oil components was normalized by peak area and expressed as a percentage. The integration method is that 0.8% of the maximum peak area was selected as the minimum integral peak. According to the above integration method, 40 peaks could be identified by comparing their real retention indices relative to n-alkanes (C₇–C₃₀) and mass spectra with the NIST 14 (National Institute of Standards and Technology, USA; 14th edition) Mass Spectral Library, which accounts for 92.03% of the total amount of essential oil compounds. The ninth peak can be determined as the mixed peak of the cis and trans isomer of the linalool oxide according to the GC-MS results of the HP-1 and HP-INNOWax capillary columns.

Table 1

Relative content and identified methods of the chemical compositions of lavender essential oil.

NO.		Compound Name	Molecular Formula	Lit. RI_HP-5^a	Exp. RI_{HP-5 ms}^b	Exp. RI_HP-1	Exp. RI_HP-INNOWax	Relative Content/% ^c	Identified Methods
1		Camphene	C₁₀H₁₆	952	943	-	1088	0.41	GC-MS, RI
2		β-Myrcene	C₁₀H₁₆	991	989	982	1168	0.60	GC-MS, RI
3		p-Cymene	C₁₀H₁₄	1025	1035	1014	1285	0.23	GC-MS, RI
4		β-Cymene	C₁₀H₁₄	1023	1021	1011	1290	0.61	GC-MS, RI
5		Limonene	C₁₀H₁₆	1030	1024	-	1217	0.58	GC-MS, RI
6		Cineole	C₁₀H₁₈O	1032	1026	1021	1219	1.05	GC-MS, RI
7		β-cis-Ocimene	C₁₀H₁₆	1038	1033	1026	1240	3.31	GC-MS, RI
8		β-trans-Ocimene	C₁₀H₁₆	1049	1044	1038	1259	1.41	GC-MS, RI
9		cis-Linalool oxide	C₁₀H₁₈O₂	1074	1069	1059	1455	0.49	GC-MS, RI, ¹³C-NMR
10		trans-Linalool oxide	C₁₀H₁₈O₂	1086	1069	1074	1484	0.49	GC-MS, RI, ¹³C-NMR
11		α-Terpinolen	C₁₀H₁₆	1088	1086	-	1301	0.63	GC-MS, RI
12		Linalool	C₁₀H₁₈O	1099	1102	1090	1545	19.71	GC-MS, RI, ¹³C-NMR
13		Hotrienol	C₁₀H₁₆O	1107	1105	-	-	0.46	GC-MS, RI, ¹³C-NMR
14		1-Pentylallyl acetate	C₁₀H₁₈O₂	1111	1114	1095	1380	1.01	GC-MS, RI
15		(4E,6Z)-allo-Ocimene	C₁₀H₁₆	1131	1128	-	-	0.25	GC-MS, RI
16		Camphor	C₁₀H₁₆O	1145	1140	1123	-	0.42	GC-MS, RI
17		(−)-Borneol	C₁₀H₁₈O	1167	1161	1152	1723	1.78	GC-MS, RI, ¹³C-NMR
18		Lavandulol	C₁₀H₁₈O	1170	1166	-	1681	0.48	GC-MS, RI, ¹³C-NMR
19		2,2,6-trimethyl-6-vinyltetrahydro-2H-pyran-3-ol	C₁₀H₁₈O₂	-	1166	-	-	-	GC-MS, RI, ¹³C-NMR
20		4-Terpineol	C₁₀H₁₈O	1177	1173	1165	1621	0.41	GC-MS, RI
21		Cryptone	C₉H₁₄O	1184	1182	1160	1715	0.62	GC-MS, RI, ¹³C-NMR
22		α-Terpineol	C₁₀H₁₈O	1189	1187	1176	1712	3.61	GC-MS, RI
23		Verbenone	C₁₀H₁₄O	1205	1204	1185	-	0.22	GC-MS, RI
24		Bornyl formate	C₁₁H₁₈O₂	1226	1225	-	-	0.41	GC-MS, RI
25		Neryl alcohol	C₁₀H₁₈O	1228	1229	1213	1804	0.49	GC-MS, RI
26		p-Cumic aldehyde	C₁₀H₁₂O	1239	1238	-	1823	0.72	GC-MS, RI
27		Linalyl acetate	C₁₂H₂₀O₂	1257	1263	1242	1564	26.61	GC-MS, RI, ¹³C-NMR
28		Phellandral	C₁₀H₁₆O	1276	1273	1254	-	0.35	GC-MS, RI
29		3,7-dimethylocta-1,7-diene-3,6-diol	C₁₀H₁₈O	-	1276	-	-	-	GC-MS, RI, ¹³C-NMR
30		Bornyl acetate	C₁₂H₂₀O₂	1285	1285	-	-	0.71	GC-MS, RI
31		Cuminol	C₁₀H₁₄O	1289	1290	-	-	0.33	GC-MS, RI
32		Lavandulol acetate	C₁₂H₂₀O₂	-	1295	1273	1612	12.68	GC-MS, RI, ¹³C-NMR
33		(E)-7-hydroxy-3,7-dimethylocta-1,5-dien-ylacetate	C₁₂H₂₀O₃	-	1344	-	-	-	GC-MS, RI, ¹³C-NMR
34		Nerol acetate	C₁₂H₂₀O₂	1364	1369	1343	1735	1.07	GC-MS, RI
35		Geranyl acetate	C₁₂H₂₀O₂	1387	1387	1361	1765	1.75	GC-MS, RI
36		β-Caryophyllen	C₁₅H₂₄	1419	1416	1418	1635	1.75	GC-MS, RI
37		α-Santalene	C₁₅H₂₄	1420	1419	-	-	0.83	GC-MS, RI
38		(E)-β-Fanesene	C₁₅H₂₄	1457	1459	1447	1672	0.49	GC-MS, RI, ¹³C-NMR
39		d-Germacrene	C₁₅H₂₄	1481	1480	1476	-	0.31	GC-MS, RI, ¹³C-NMR
40		γ-Cadinene	C₁₅H₂₄	1513	1514	1509	-	0.23	GC-MS, RI, ¹³C-NMR
41		11-hydroxy-α-santal-9-ene	C₁₅H₂₄O	-	1519	-	-	-	GC-MS, RI, ¹³C-NMR
42		2-Methyl-1-(4-methylphenyl)-3-buten-1-ol	C₁₂H₁₆O	-	1519	-	-	0.52	GC-MS,RI
43		β-Caryophyllene oxide	C₁₅H₂₄O	1581	1585	1571	2042	3.65	GC-MS, RI, ¹³C-NMR
44		Cedrelanol	C₁₅H₂₆O	1640	1643	1628	2206	0.89	GC-MS,RI
45		(3S,6R,9R)-2-(hydroxymethyl)-5,5,9-trimethyltricyclo [7.2.0.0(3,6)]undecan-2-ol	C₁₅H₂₆O₂	-	1811	-	-	-	GC-MS, RI, ¹³C-NMR
46		(6R,10R)-6,10,14-Trimethyl-2-pentadecanone	C₁₈H₃₆O	-	1846	-	-	-	GC-MS, RI, ¹³C-NMR
47		S₈		-	2031	-	-	-	GC-MS
Component group
Monoterpene hydrocarbons								8.03
Sesquiterpene hydrocarbons								3.61
Oxygenated monoterpenes								31.53
Oxygenated sesquiterpenes								4.54
	Esters							43.23
	Others							1.14
	Total identified							92.08

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Bold type indicates major component. The blue type represents the newly identified compound. ^a Literature retention indices (HP-5 column) according to NIST 14 (National Institute of Standards and Technology, USA; 14th edition) library database (https://webbook.nist.gov); ^b Experiment retention indices (HP-5 ms column); ^c Relative abundance calculated on HP-5 ms capillary column.

Forty compounds were identified in the essential oil by GC-MS, representing 92.03% of the total volatiles (Table 1). The identified monomers belong to different chemical classes and are present in different proportions. The essential oil contains essentially oxygenated monoterpenes (31.53%) and esters (43.23%), significant fraction monoterpene hydrocarbons (8.03%), sesquiterpene hydrocarbons (3.61%), oxygenated sesquiterpenes (4.54%), and small quantities of other compounds (1.14%). Linalool (12, 19.71%)—an oxygenated monoterpene—and linalyl acetate (27, 26.61%) and lavandulol acetate (32, 12.68%)—ester compounds—were the main components of the lavender essential oil.

The compounds that were separated by silica gel column chromatography and pGC were analyzed and identified by NMR (Figures S4–S21) (Supplementary Materials). Twelve compositions, which were compounds 9, 10, 12, 13, 17, 18, 21, 27, 32, 38, 39, 40 and 43, were further identified by comparing the carbon spectrum data in the literature [16,17,18,19,20,21,22,23,24,25,26,27,28]. Compared with the method of determining compounds by GC-MS, six missing compositions, which were compounds 19, 29, 33, 41, 45 and 46, were identified by comparing the carbon spectrum data in the literature [29,30,31,32,33,34], and could not be retrieved from the NIST 14 database. By comparison with the literature [35], 15 compounds were newly identified. In this study, sulfur was isolated from essential oil for the first time, and was identified by GC-MS, whose mass spectrogram is shown in Figures S2 and S3 (Supplementary Materials). Therefore, it was proved that lavender essential oil contained a small amount of sulfur compounds. The sulfur compounds provide a new explanation for the insecticidal activity of lavender essential oil and provide a new research idea for later researchers. This finding may help to develop lavender essential oil into a new natural acaricides [36].

2.2. Improvements in the Fraction Collector of pGC

In the early stages of the separation of lavender essential oils by pGC, we found that the cost and separation efficiency of this instrument were too low. To solve this problem, we converted the liquid nitrogen cooling system of the fraction collector to a cold trap cooling system. This improvement not only increased efficiency but also greatly reduced costs.

By improving the preparation fraction collector of pGC without affecting the normal operation of the pGC, it was found that the improved pGC had a similar collection amount in the same time and temperature compared with the unmodified equipment. The liquid nitrogen cooling system of the preparative fraction collector of pGC was transformed into a cold well cooling system. This improvement doubled the efficiency of preparative gas chromatography, and the cost of the cooling system was reduced from RMB 675/day to RMB 25/day.

3. Materials and Methods

3.1. Plant Material and Reagents

The aerial parts of Lavandula angustifolia Mill. were collected in June 2019 in Yili, Xinjiang, China. The plant material was dried in the shade. The plant sample was identified by Dr. Chunfang Lu and the voucher specimen was stored in the Xinjiang Technical Institute of Physics and Chemistry, Urumqi, Chinese Academy of Sciences (voucher species man No. WY02260). Anhydrous sodium sulfate was purchased from Tianjin Hongyan chemical reagent factory (Tianjin, China), n-hexane, ethyl acetate, and acetic acid were purchased from Tianjin Yongsheng Superfine Chemical Industry Co., Ltd. (Tianjin, China). n-Alkanes (C₇–C₃₀) were purchased from Sigma-Aldrich (Shanghai, China).

3.2. Extraction of the Essential Oil

The lavender essential oil was obtained from aerial parts of the lavender that were cut into about 2 cm by hydrodistillation for 3 h using a self-assembling Clevenger-type apparatus, and the ratio of material to liquid was 1:10. The essential oil was collected, centrifuged, and taken from the upper layer. The lavender essential oil was extracted three times with 1200 g of material each time. The yields were 12.9852 g, 13.9137 g and 11.5129 g, respectively. The upper layer (essential oil) was dried over Na₂SO₄ and stored at −20 °C until required for analysis and separation.

3.3. Gas Chromatography Analysis

The analysis of the essential oil was first performed on an Agilent GC-QTOF-MS system consisting of a 7890B gas chromatograph equipped with HP-5 ms capillary column (30 m × 0.25 mm i.d., film thickness 0.25 µm) and 7693 autosampler, connected to a hybrid QTOF mass spectrometer (Agilent model 7200, Santa Clara, CA, USA), controlled by MassHunter Acquisition B.07.00 software (Santa Clara, CA, USA).

Helium was used as a carrier gas at a flow rate of 1 mL/min. The injector and detector temperatures were 250 °C. The oven temperature was programmed from 70 (10 min) to 100 °C at a rate of 2 °C/min, then from 100 to 200 °C at a rate of 5 °C/min. The injection volumes were 0.4 µL. The split injection was conducted with a split ratio of 1:40. The mass spectra were recorded at 70 eV (EI) and were scanned in the range 50–500 m/z.

The essential oil was analyzed on the Agilent GC-MS system consisting of a 7693 autosampler and 7890A gas chromatograph connected to a 5975C mass spectrometer (Santa Clara, CA, USA) (inert XL EI/CI MSD with Triple-Axis detector), controlled by 5975-7890GC-MS software (Santa Clara, CA, USA). The GC was equipped with HP-INNOWax (30 m × 0.25 mm i.d., film thickness 0.25 µm) and HP-1 capillary columns (30 m × 0.25 mm i.d., film thickness 0.25 µm). Nitrogen was used as a carrier gas at a flow rate of 0.8 mL/min. The injector and detector temperatures were 250 °C. The oven temperature was programmed from 60 to 180 °C at a rate of 8 °C/min, then from 180 to 240 °C (5 min) at a rate of 10 °C/min. The injection volumes were 0.8 µL. The split injection was conducted with a split ratio of 1:40. The mass spectra were recorded at 70 eV (EI) and were scanned in the range 30–500 m/z. The components were identified by comparing their real retention indices relative to the n-alkanes (C₇–C₃₀) and the mass spectra with the NIST 14 Mass Spectral Library. The formula for calculating the retention index is as follows [36]:

$RI = 100 Z + 100 (T_{x} - T_{z}) / (T_{z + 1} - T_{z})$

(1)

where T_X is the component retention time; Z is the carbon number of n-alkanes component; T_Z is the the retention time of n-alkanes that carbon number is Z; and T_Z+1 is the the retention time of n-alkanes that carbon number is Z + 1.

3.4. Isolation and Structure Elucidation

The pGC was modified based on the Agilent 7890B gas chromatograph system (Agilent model 7200, Santa Clara, CA, USA). It was equipped with a HP-5 capillary column (30 m × 0.53 mm i.d., film thickness 1.0 µm), a G4513A autosampler, a flame ionization detector (FID), a modular analytical system and a preparative fraction collector (Gerstel Company, Mülheim, Germany) equipped with a home-made cold trap cooling system.

In brief, 30.0 g of essential oil was firstly separated by silica gel column chromatography. The separation conditions were 380 g, 200–300 mesh silica gel and 60 × 600 mm column volume. The elution gradients were n-hexane:ethyl acetate = 100:0, 100:1, 100:1.5, 100:3, 100:8, 100:15, 100:30, 100:50, 0:100. According to the results of the thin layer, divided appropriately, the fractions were condensed to 4 mL and stored in brown vials at −20 °C. Then, suitable fractions were selected to separate monomers by improved pGC. Seven fractions were selected to separate the compounds by pGC, which were successively named A-G. Compound 38 (3.0 mg), compound 39 (1.3 mg), and compound 40 (0.8 mg) were prepared from A fractions by pGC. The injection volumes were 0.5 µL. The number of injections was 466. The oven temperature of pGC was programmed from 70 to 160 °C at a rate of 50 °C/min, then from 160 to 174 °C at a rate of 2 °C/min, and then from 174 to 280 °C (0.5 min) at a rate of 50 °C/min. Compounds 13 (1.5 mg), 18 (1.9 mg), 21 (3.7 mg) and 41 (0.7 mg) were prepared from B fractions by pGC. The injection volumes were 0.5 µL. The number of injections was 411. The oven temperature of pGC was programmed from 70 to 150 °C at a rate of 10 °C/min, then from 150 to 250 °C (0.5 min) at a rate of 50 °C/min. Compounds 27 (25.7 mg), 32 (15.3 mg), 43 (3.5 mg) and 46 (1.4 mg) were prepared from C fractions by pGC. The injection volumes were 1.0 µL. The number of injections was 524. The oven temperature of pGC was programmed from 100 to 170 °C at a rate of 10 °C per min, then from 170 to 250 °C (1.5 min) at a rate of 30 °C/min. Compounds 18 (3.8 mg), 17 (4.2 mg) and 41 (0.3 mg) were prepared from D fractions by pGC. The injection volumes were 1.0 µL. The number of injections was 435. The oven temperature of pGC was programmed from 150 (5 min) to 230 °C at a rate of 20 °C/min (1.5 min). Compounds 10 (5.2 mg) and 33 (2.7 mg) were prepared from E fractions by pGC. The injection volumes were 1.0 µL. The number of injections was 376. The oven temperature of pGC was programmed from 140 to 150 °C at a rate of 2 °C/min, then from 150 to 180 °C at a rate of 10 °C/min, then from 180 to 280 °C (5 min) at a rate of 100 °C/min. Compound 29 (27.5 mg) was prepared from F fractions by pGC. The injection volumes were 5.0 µL. The number of injections was 241. The oven temperature of pGC was programmed from 100 to 240 °C (1.5 min) at a rate of 20 °C/min. Compound 45 (1.0 mg) was prepared from G fractions by pGC. The injection volumes were 4.0 µL. The number of injections was 431. The oven temperature of pGC was programmed from 100 to 200 °C at a rate of 25 °C/min, then from 200 to 250 °C (3 min) at a rate of 20 °C/min. The structure of the monomers was proved by ¹³C-NMR.

4. Conclusions

In this study, the chemical composition of the lavender essential oil obtained from the aerial parts was studied by pGC and GC-MS equipped with three capillary columns of different polarity for the first time. Using GC-MS equipped with three capillary columns to analyze lavender essential oil made the composition accuracy of lavender essential oil more valuable for reference. Compared with the method of determining compounds by GC-MS, six missing compositions, which were 2,2,6-trimethyl-6-vinyltetrahydro-2H-pyran-3-ol, 3,7-dimethylocta-1,7-diene-3,6-diol, (E)-7-hydroxy-3,7-dimethylocta-1,5-dien-ylacetate, 11-hydroxy-α-santal-9-ene, (3S,6R,9R)-2-(hydroxymethyl)-5,5,9-trimethyltricyclo[7.2.0.0(3,6)]undecan-2-ol and (6R,10R)-6,10,14-Trimethyl-2-pentadecanone, were identified. In comparison with the literature about lavender essential oil, 15 compounds were newly identified. The improved pGC not only doubled the efficiency but also greatly reduced the cost. This improved pGC also holds great promise.

Acknowledgments

This research was funded by the Recruitment Program of Global Experts awarded to Maiwulanjiang, and the Director Foundation of XTIPC, CAS (2016RC002).

Supplementary Materials

The supplementary data consist of the Figures S1–S21 and spectral data. Figure S1 showed GC-MS equipped with HP-5 ms, HP-1 and HP-INNOWax capillary columns TiC spectra for Lavender essential oil. Figures S2–S21 showed GC-EI-QTOF-MS Tic spectra, MS spectra and ¹³C spectra for compounds 9, 10, 12, 13, 17, 18, 19, 21, 27, 29, 32, 33, 38, 39, 40, 41, 43, 45, 46, 47.

Click here for additional data file.^{(3.3M, pdf)}

Author Contributions

Conceptualization, H.A.A. and M.M.; Methodology, G.D.; investigation, G.D.; resources, A.A., X.B.; project administration, M.M.; supervision, M.M.; funding acquisition, H.A.A. and M.M.; writing—original draft, G.D.; writing—review & editing, M.M. All authors have read and approved the manuscript.

Funding

This research was funded by the Recruitment Program of Global Experts awarded to Maiwulanjiang, and the Director Foundation of XTIPC, CAS (2016RC002).

Conflicts of Interest

The authors declare no conflict of interest.

Footnotes

Sample Availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.

References

1. Tabata J., Teshiba M., Shimizu N., Sugie H. Mealybug mating disruption by a sex pheromone derived from lavender essential oil. J. Essent. Oil Res. 2015;27:232–237. doi:10.1080/10412905.2015.1007219. [CrossRef] [Google Scholar]

2. Maria L.-B. Lavender The Genus Lavandula. Taylor & Francis; London, UK: New York, NY, USA: 2002. pp. 2–86. [Google Scholar]

3. Lubbe A., Verpoorte R. Cultivation of medicinal and aromatic plants for specialty industrial materials. Ind. Crop. Prod. 2011;34:785–801. doi:10.1016/j.indcrop.2011.01.019. [CrossRef] [Google Scholar]

4. Imane M.M., Houda F., Amal A.H.S., Kaotar N., Mohammed T., Imane R., Farid H. Phytochemical composition and antibacterial activity of moroccan Lavandula angustifolia mill. J. Essent. Oil. Bear. Plants. 2017;20:1074–1082. doi:10.1080/0972060X.2017.1363000. [CrossRef] [Google Scholar]

5. Bialon M., Krzysko-Lupicka T., Nowakowska-Bogdan E., Wieczorek P.P. Chemical composition of two different lavender essential oils and their effect on facial skin microbiota. Molecules. 2019;24:3270. doi:10.3390/molecules24183270. [PMC free article] [PubMed] [CrossRef] [Google Scholar]

6. Ali-Shtayeh M.S., Abu-Zaitoun S.Y., Dudai N., Jamous R.M. Downy lavender oil: A promising source of antimicrobial, antiobesity, and anti-alzheimer’s disease agents. Evid.-Based Complementary Altern. 2020;2020:5679408. doi:10.1155/2020/5679408. [PMC free article] [PubMed] [CrossRef] [Google Scholar]

7. Al-Sarar A.S., Hussein H.I., Abobakr Y., Bayoumi A.E., Al-Otaibi M.T. Fumigant toxicity and antiacetylcholinesterase activity of Saudi Mentha longifolia and Lavandula dentata species against Callosobruchus maculatus (F.)(Coleoptera: Bruchidae) Turk. Entomoloji Derg-Turk. 2014;38:11–18. doi:10.1051/acarologia/20142118. [CrossRef] [Google Scholar]

8. Romeo F.V., De Luca S., Piscopo A., Poiana M. Antimicrobial effect of some essential oils. J. Essent. Oil Res. 2008;20:373–379. doi:10.1080/10412905.2008.9700034. [CrossRef] [Google Scholar]

9. Tomi K., Kitao M., Murakami H., Matsumura Y., Hayashi T. Classification of lavender essential oils: Sedative effects of Lavandula oils. J. Essent. Oil Res. 2018;30:56–68. doi:10.1080/10412905.2017.1377122. [CrossRef] [Google Scholar]

10. Cavanagh H.M.A., Wilkinson J.M. Biological activities of Lavender essential oil. Phytother. Res. 2002;16:301–308. doi:10.1002/ptr.1103. [PubMed] [CrossRef] [Google Scholar]

11. Sharifi-Rad J., Sureda A., Tenore G.C., Daglia M., Sharifi-Rad M., Valussi M., Tundis R., Sharifi-Rad M., Loizzo M.R., Ademiluyi A.O. Biological activities of essential oils: From plant chemoecology to traditional healing systems. Molecules. 2017;22:70. doi:10.3390/molecules22010070. [PMC free article] [PubMed] [CrossRef] [Google Scholar]

12. Carrasco A., Martinez-Gutierrez R., Tomas V., Tudela J. Lavandula angustifolia and lavandula latifolia essential oils from spain: Aromatic profile and bioactivities. Planta Med. 2015;82:163–170. doi:10.1055/s-0035-1558095. [PubMed] [CrossRef] [Google Scholar]

13. Woronuk G., Demissie Z., Rheault M., Mahmoud S. Biosynthesis and therapeutic properties of lavandula essential oil constituents. Planta Med. 2011;77:7–15. doi:10.1055/s-0030-1250136. [PubMed] [CrossRef] [Google Scholar]

14. Adenubi O.T., Ahmed A.S., Fasina F.O., Mcgaw L.J., Eloff J.N., Naidoo V. Pesticidal plants as a possible alternative to synthetic acaricides in tick control: A systematic review and meta-analysis. Ind. Crop. Prod. 2018;123:779–806. doi:10.1016/j.indcrop.2018.06.075. [CrossRef] [Google Scholar]

15. Wojtunik-Kulesza K.A., Targowska-Duda K., Klimek K., Ginalska G., Jozwiak K., Waksmundzka-Hajnos M., Ciesla L. Volatile terpenoids as potential drug leads in Alzheimer’s disease. Open Chem. 2017;15:332–343. doi:10.1515/chem-2017-0040. [CrossRef] [Google Scholar]

16. Francisco C.G., Freire R., Hernández R., Salazar J.A., Suárez E., Cortés M. Carbon-13 NMR study of (20,24)-epoxydammarane triterpenes. Magn. Reson. Chem. 1984;22:34–38. doi:10.1002/mrc.1270220108. [CrossRef] [Google Scholar]

17. Shin S.W., Choi S.H., Lim S. Combined Effects of the Essential Oil from Pelargonium graveolens with Antibiotics against Streptococcus pneumonia. Nat. Prod. Sci. 2007;13:342–346. [Google Scholar]

18. Schulz S., Steffensky M., Roisin Y. Identification and synthesis of elymniafuran, a new monoterpene from the butterfly elymnias thryallis. Liebigs Ann. 1996;6:941–946. doi:10.1002/jlac.199619960612. [CrossRef] [Google Scholar]

19. Choi J.W., Kim K.H., Lee I.K., Choi S.U., Lee K.R. Phytochemical constituents of amomum xanthioides. Nat. Prod. Sci. 2009;15:44–49. [Google Scholar]

20. Miyazawa M., Nankai H., Kameoka H. Enantioselective cyclization of (+/-)-lavandulol to (-)-(2S,4S)-1,5-epoxy-5-methyl-2-(1-methylethenyl)-4-hexanol by Glomerella cingulate. Nat. Prod. Lett. 1997;9:249–252. doi:10.1080/10575639708043635. [CrossRef] [Google Scholar]

21. Queiroga C.L., Ferracini V.L., Marsaioli A.J. Three new oxygenated cadinanes from baccharis species. Phytochemistry. 1996;42:1097–1103. doi:10.1016/0031-9422(95)00977-9. [CrossRef] [Google Scholar]

22. Guan J., Zhao W.J., Wei J.J., Gao L. Studies on the chemical constituents of the extracts of lavender flowers from supercritical CO₂ fluid. Lishizhen Med. Mater. Med. 2009;20:890–891. doi:10.3969/j.issn.1008-0805.2009.04.065. [CrossRef] [Google Scholar]

23. Bohlmann F., Zeisberg R., Klein E. 13C-NMR-spektren von monoterpenen. Magn. Reson. Chem. 2005;7:426–432. doi:10.1002/mrc.1270070907. [CrossRef] [Google Scholar]

24. Srivastava P.L., Daramwar P.P., Krithika R., Pandreka A., Shankar S.S., Thulasiram H.V. Functional characterization of novel sesquiterpene synthases from indian sandalwood, santalum album. Sci. Rep. 2015;5:10095. doi:10.1038/srep10095. [PMC free article] [PubMed] [CrossRef] [Google Scholar]

25. Umlauf D., Zapp J., Becker H., Adam K.P. Biosynthesis of the irregular monoterpene artemisia ketone, the sesquiterpene germacrene D and other isoprenoids in Tanacetum vulgare L. (Asteraceae) Phytochemistry. 2004;65:2463–2470. doi:10.1016/j.phytochem.2004.08.019. [PubMed] [CrossRef] [Google Scholar]

26. Burkhardt I., Kreuzenbeck N., Beemelmanns C., Dickschat J. Mechanistic characterization of three sesquiterpene synthases from the termiteassociated fungus Termitomyces. Org. Biomol. Chem. 2019;17:3348–3355. doi:10.1039/C8OB02744G. [PubMed] [CrossRef] [Google Scholar]

27. Ragasa C.Y., Ganzon J., Hofilena J., Tamboong B., Rideout J.A. A new furanoid diterpene from Caesalpinia pulcherrima. Chem. Pharm. Bull. 2003;51:1208–1210. doi:10.1248/cpb.51.1208. [PubMed] [CrossRef] [Google Scholar]

28. Weinert B., Wüst M., Mosandl A., Hanssum H. Stereoisomeric flavour compounds. LXXVIII. Separation and structure elucidation of the pyranoid linalool oxide stereoisomers using common gas chromatographic phases, modified cyclodextrin phases and nuclear magnetic resonance spectroscopy. Phytochem. Anal. 1998;9:10–13. doi:10.1002/(SICI)1099-1565(199801/02)9:1<10::AID-PCA382>3.0.CO;2-D. [CrossRef] [Google Scholar]

29. Strauss C.R., Wilson B., Rapp A., Guentert M., Williams P.J. New monoterpene ethyl ethers in grape wines and brandies. J. Agric. Food Chem. 1985;33:706–708. doi:10.1021/jf00064a034. [CrossRef] [Google Scholar]

30. Mookherjee B.D., Trenkle R.W. Isolation, identification, and biogenesis of bifunctional compounds in lavandin oil. J. Agric. Food Chem. 1973;21:298–302. doi:10.1021/jf60186a011. [CrossRef] [Google Scholar]

31. Ngo K.S., Brown G.D. Santalane and isocampherenane sesquiterpenoids from illicium tsangii. Phytochemistry. 1999;50:1213–1218. doi:10.1016/S0031-9422(98)00652-9. [CrossRef] [Google Scholar]

32. Singh D., Kumar Chaudhuri P., Darokar M.P. New antiproliferative tricyclic sesquiterpenoid from the leaves of Ocimum sanctum. Helv. Chim. Acta. 2014;97:708–711. doi:10.1002/hlca.201300269. [CrossRef] [Google Scholar]

33. Gao Y., Yang W.Q., Wang F., Liu J.K., Gao J.M. Chemical component from allelopathic cultivar sunflower leaves. Acta Bot. Boreali-Occident. Sin. 2007;27:532–536. doi:10.1016/S1872-2075(07)60055-7. [CrossRef] [Google Scholar]

34. De Andrade D.J., Pattaro F.C., De Oliveira C.A.L. Lime sulfur residue on acaricide efficiency in the control of Brevipalpus phoenicis. Cienc. Rural. 2011;41:1695–1701. doi:10.1590/S0103-84782011005000129. [CrossRef] [Google Scholar]

35. Adaszynska-Skwirzynska M., Dzieciol M. Comparison of chemical composition and antimicrobial activity of essential oils obtained from different cultivars and morphological parts of Lavandula angustifolia. J. Essent. Oil Bear. Plants. 2018;21:1532–1541. doi:10.1080/0972060X.2018.1526123. [CrossRef] [Google Scholar]

36. Vandendool H., Kratz P.D. A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J. Chromatogr. A. 1963;11:463–471. doi:10.1016/S0021-9673(01)80947-X. [PubMed] [CrossRef] [Google Scholar]

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Study on Lavender Essential Oil Chemical Compositions by GC-MS and Improved pGC (2024)

FAQs

What is the chemical composition of lavender essential oil? ›

According to the European Pharmacopoeia, the relative content of lavender oil compounds should be in the following ranges: limonene (maximum 1.0%), 1,8-cineole (maximum 2.5%), 3-octanone (0.1–5.0%), camphor (maximum 1.2%), linalool (20.0–45.0%), linalyl acetate (25.0–47.0%), terpinen-4-ol (0.1–8.0%), lavandulyl acetate ...

What is the difference between lavender essential oil and lavender 40 42 essential oil? ›

Slight medicinal aroma compared to Lavender (30/32). The 40/42 in the name Lavender 40/42 means that it has been standardized by blending with a variety of lavender and lavadin essential oils to make up the aromatic lavender notes of 40% linalool and 42% linalyl acetate.

What are the side effects of lavender essential oils? ›

Using lavender essential oils directly with food or drinks can be dangerous for people. Consuming essential oils directly causes irritation to the oral mucosa, lining of the soft palate, throat, and tongue. Other side effects include constipation, headache, increased appetite and skin rash on topical use.

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Does lavender increase estrogen? ›

The oil was inhaled in a study evaluating salivary estrogen levels in peri-menopausal women and researchers concluded that it does not have an effect on estrogen levels.

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How can you tell if an essential oil is 100% pure? ›

As much as you might want to believe you can tell if your oils are pure essential oils based on the aroma or the energy, it's impossible to know if your essential oils are pure without lab testing. The most reliable laboratory test is called GC/MS which stands for “Gas Chromatography/Mass Spectrometry.”